Purified proteins have many different uses and applications, from food to medicine. Its role in detecting tuberculosis and its use as a nutritional supplement is of high value
Source: Flickr user Melissa Wiese
Protein measurement is necessary for these various medical applications, depending on the precise calculation of absorbance values using a spectrophotometer. The ability to quantify protein concentration levels provides the necessary information for protein measurement analysis and use. Researchers Using Protein Measurement Methods for Purifying Protein Derivatives and Multiple Protein Assays The spectrophotometer was designed to obtain protein measurements and provide convenient and accurate results.
Protein measurement and analysis
Proteins are a readily available resource that can be obtained from a variety of sources. Proteins can be derived from plant and animal products and used for various purposes. The protein measurements for these products are required to meet the strict FDA (Food and Drug Administration) daily value percentage (DV%) and properly labeled guidelines. The spectrophotometer measures absorbance values from the intrinsic chromophores tryptophan, tyrosine and cystine (the cysteine in oxidized disulfide bonds) to determine the level of protein concentration in a given sample. Protein measurements with low levels of absorbance can be very difficult to detect, and advanced spectrophotometry can only be quantified by using light absorption measurements.